FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_wt_n_3.35100000040939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_wt_n_3.35100000040939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14216647
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237160.16681851916278148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT13250.010.3709151
CGGGGAT16050.09.965211
CGCGGAT9950.09.9616831
CGCCCAT12900.09.7791591
GGGGAAT34250.09.6685181
CCCGGAT17300.09.6358221
CGCGGGG2851.734974E-59.4850481
GCACACG14250.09.3151211
GGGGACT22250.09.314531
CCCGGGT10750.09.2203491
CGGGGAG15200.09.1886411
CTGGAAT62200.08.6196521
GGGGAAC19450.08.570681
GGGGATT32500.08.45628451
CGGGGGT6401.4551915E-118.4476211
GGAAGAT120250.08.1490561
CGGGTAT13700.08.0570981
CGTATGC15650.08.04949244
GTCGGAT26650.08.0302591
CGGGAAT25750.07.96099421
Produced by FastQC (version 0.11.2)