FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_wt_n_2.351000000408da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_wt_n_2.351000000408da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14068483
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163060.11590446532152757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT11400.010.8674671
CGGGGGG3959.640644E-1110.8349521
CGCCCTT10550.010.2484751
GGGGAAT33550.09.9366431
CTGGGAT37600.09.76495551
CGGGAAT24350.09.7131651
CGGGGAT15600.09.2411461
CGGGACT11300.08.7709111
CTCGAAT34100.08.6534131
GGGAGAT56250.08.64971351
CGGGGGT7150.08.5060561
CGGGGAG14600.08.4855571
CTGGAAT60350.08.4726471
GGGGACT23550.08.4170951
CTCGGAT41250.08.300231
CGGGCAT13850.08.2945131
CGCCTAT7551.8189894E-128.0554031
CCCGACT18050.07.9868081
CCCGGAT18300.07.8776991
CCCGCAT9750.07.8549751
Produced by FastQC (version 0.11.2)