FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_wt_n_1.35100000040860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_wt_n_1.35100000040860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17966420
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214020.11912222913635548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT19700.010.4057161
CGCCCTT14050.09.9405891
CGCGCAT6400.09.5034351
GGGGAAT44050.09.4606851
CGGGCAT19150.09.4106151
CTGGGAT48550.09.2333781
CGGGGGG4304.7701178E-88.9058891
CTCGAAT44200.08.8169961
GGGGAAC25250.08.6538061
CTCGGGT30050.08.6208511
CCGGGGT7100.08.5664761
CCCGGAT24650.08.4989021
CGGGCTT23450.08.4535011
CGCGGAT13100.08.4260021
CGGGGAC12050.08.4124451
CGGGAAT31400.08.3936911
GGGGATT45600.08.2992951
GGGAGAT69600.08.2533021
GGGGACT30050.08.2460321
CTCGGAT51200.08.227511
Produced by FastQC (version 0.11.2)