FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_wt_k_3.35100000040aa2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_wt_k_3.35100000040aa2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16225477
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199410.12289931445466905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17100.010.92055111
CGGGAAT29950.010.5297041
GGGGAAT35850.010.430471
CGGGCAT15550.010.1403421
CGTATGC17750.010.01186644
CGGGGAT17250.09.6633471
GTCACGC19650.09.39423829
CGGGGAG16750.09.2793861
CACACGT21450.09.12539912
ACGTCTG20150.09.04232715
CTGGAAT69300.08.8413921
CTGGGAT44500.08.6054551
GGGAGAT65750.08.5992971
CACGCCA20450.08.5864131
CGCCCAT14850.08.4946711
CTCGCAT25150.08.4192771
GTATGCC22650.08.24320745
CTCGGAT49550.08.2284851
TCACGCC22550.08.1861130
CCCGGAT20650.08.1813731
Produced by FastQC (version 0.11.2)