FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_wt_k_2.35100000040a2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_wt_k_2.35100000040a2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16107216
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229130.14225301256281656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT11650.012.568381
CGGGGAT17900.010.0676661
CGGGCAT17550.09.7550241
GGGGAAT38850.09.5671981
CTGGAAT72550.09.0664511
GGGGTAT9900.08.6464991
CTGGGAT44850.08.5886621
CTCGAAT38000.08.5363221
CGGGCGC4803.1994205E-88.43565415
CTCGGAT47300.08.2866691
GGGAGAT62400.08.2669021
GTCCGCT7700.08.191421
GCGCGCC4457.7792356E-78.08811819
CGGGGAG15600.08.0864021
CGCCCTT13650.08.0864021
CCGGGAT21850.08.0414631
CGTATGC14650.07.984724544
CGGGAAT28600.07.95512871
GGGAAAT56500.07.93407871
GCGGGCG4853.3830474E-77.884872414
Produced by FastQC (version 0.11.2)