FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_wt_k_1.351000000409bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_wt_k_1.351000000409bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11533851
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173570.15048746511464384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC2552.0063453E-913.23240815
CGGGGAT12700.010.8195981
GGGGGAT10050.010.758711
CGCCCAT10200.010.379651
CCCGAAT13500.010.1784361
CGCCCTT9650.010.0375141
CGGGAAT20350.09.7409941
GGGGAAT27250.09.589071
GCGGGCG3806.9645466E-89.47161914
CGCCTAT5058.913048E-119.3672681
CTGGGAT29150.09.1958831
GGGAGAT46150.08.8347021
CTCGAAT28200.08.78675651
GCACACG10250.08.77986611
CGGGCAT11850.08.7442891
CTGGAAT50550.08.6450121
CGGGGAG11850.08.3641021
GGCAGAT26550.08.2298561
CGGGGTT15150.08.1777731
CGGGGGA7601.8189894E-128.0026751
Produced by FastQC (version 0.11.2)