FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_oe_n_3.35100000040bf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_oe_n_3.35100000040bf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16432432
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308230.18757418256774164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17750.011.40602844
GCACACG18350.010.91208311
GGGGGAT14900.010.5835541
CGGGGAG14150.010.3484821
CGGGCAT16450.010.2710571
CTGGGAT46550.09.6789911
CGCCCTT12650.09.4385461
CGGGGAT19100.09.3178011
CGGGAAT28650.09.2784871
GGGAGAT66150.09.0247631
GGGGAAT34450.09.02421951
AGTCACG25550.08.89839428
CACACGT23650.08.75206312
CCCGCAT10700.08.6321671
GTATGCC24750.08.5436445
TCGTATG25200.08.39107443
CTCGGAT49600.08.3116871
CGGGGGA10050.08.2938361
GGGGGGT5201.3853423E-88.2313311
CGCCCAT18200.08.169441
Produced by FastQC (version 0.11.2)