FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_oe_n_2.35100000040b87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_oe_n_2.35100000040b87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15793680
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297600.1884298023006671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC15100.011.91803644
GGGGGAT13050.010.8741871
GCACACG17400.010.60284111
CGGGCAT14450.010.6000491
CACACGT20850.09.49579912
CGGGGAT18400.09.3038471
CGGGAAT26500.09.1800421
GGGAGAT64700.09.1562631
CTGGGAT45250.09.1095991
CCCCGCT8300.08.9557641
CCCGCAT12350.08.5723071
GGCCGAT15050.08.5311021
CCCGGGT11100.08.5230121
CTCGGAT46750.08.4800391
CTCGGGT26050.08.4739341
GCGCCCA6155.820766E-118.41268734
GGGGACT21450.08.400971
CTGGAAT71900.08.2706921
GGGGAAT36800.08.2632861
ACGTCTG24250.08.255795515
Produced by FastQC (version 0.11.2)