FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_oe_n_1.35100000040b00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_oe_n_1.35100000040b00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15614641
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265470.17001351488004113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT14250.012.1715351
CGCCCCT6000.010.511781
CGCCCTT11150.010.5050461
GCACACG16850.010.41492711
CGCGGGG2657.3197443E-610.200111
CCCGGGT9950.010.1872951
CGGGGAG13500.010.0112181
GGGGAAT35400.09.9263781
CGTATGC17600.09.84172944
CTCGAAT39100.09.4479281
CCCGAAT17350.09.0879941
CGGGAAT26950.08.9432331
CTGGGAT42400.08.8719711
CTGGAAT69800.08.6486611
CTGGCAT25000.08.4694911
CGCGCAC2952.611009E-48.3992431
GTATGCC22250.08.39153545
CGGGGAT16200.08.3426831
GGGAGAT62550.08.1746291
ACGTCTG23700.08.16269715
Produced by FastQC (version 0.11.2)