FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_oe_k_3.35100000040cf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_oe_k_3.35100000040cf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14539454
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198630.13661448359752712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17850.015.12496811
CGTATGC17750.014.82793644
CACACGT21200.012.41647912
ACGTCTG22200.011.95667115
ACACGTC22550.011.772305513
GTATGCC22550.011.77141545
CGCCCTT11850.010.4550211
TCGTATG25800.010.28865243
CACGTCT27600.010.106338514
GGGGGAT13000.09.7034291
CTCGAAT33900.09.5020411
CTGGAAT64100.09.4531121
CTGGGAT38800.09.3470541
CGTCTGA30750.09.29051616
GGGAGAT59300.09.192661
CGGGGAT17400.09.1915691
CTCGTAT28450.09.09309642
AGCACAC31700.09.08452310
CGGGAAT24800.08.7196711
GGGGAAT33350.08.442961
Produced by FastQC (version 0.11.2)