FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_oe_k_2.35100000040cbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_oe_k_2.35100000040cbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14612010
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262340.17953724367831667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18650.013.99170844
GCACACG18350.013.73197511
ACGGCTA22150.012.0919232
CACACGT21100.012.04876612
ACGTCTG23200.011.92601615
CGGGGAT13550.011.8033961
CGGCTAC22150.011.78207933
GTATGCC22900.011.49322745
TCGTATG24650.010.95109143
AGTCACG22850.010.73869628
CACGGCT24150.010.62671231
CGCCTAT6250.010.4521571
ACACGTC24700.010.11032613
CTGGGAT36850.09.902971
GTCACGG26450.09.5324329
GGGAGAT56450.09.4973171
GGGGAAT32500.09.0797911
CGGGCGC3755.8271144E-78.99783915
CACGTCT28300.08.98194114
CGTCTGA29950.08.86264616
Produced by FastQC (version 0.11.2)