FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l03n01_js_oe_k_1.35100000040c50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l03n01_js_oe_k_1.35100000040c50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15160925
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234630.15475968649670124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT12100.012.2871771
CGGGGAT18600.011.8687971
GCACACG16400.011.38646311
CGTATGC16150.010.86474144
CTGGGAT41150.09.1419791
CGGGCGC3206.949438E-69.13865115
CTCGGAT44600.09.0914091
CACACGT21700.09.02015812
GTATGCC20200.08.90914345
CTGGAAT66800.08.9026851
GGGAGAT62150.08.5901511
CGGGAAT27100.08.5617291
GGGGTAT11150.08.4853141
CTCGGTT40700.08.468191
CCCGCAT10650.08.4606541
ACGTCTG22750.08.40477815
CTCGAAT37950.08.3101671
CGCGGAT12500.08.2897491
CCCGGCT13500.08.1762821
CGCCCTT11150.08.0812521
Produced by FastQC (version 0.11.2)