FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l02n01_sd2_3_smallrna.3510000004092c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l02n01_sd2_3_smallrna.3510000004092c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43023126
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG717708916.681932874891515Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGATGGAATT22366255.198657577787351No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA16724483.887323296777645RNA PCR Primer, Index 1 (100% over 22bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG13148283.056095923852674Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG9760692.2687077642847244No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA9536382.2165706880527463RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC6253861.4536042778481508RNA PCR Primer, Index 1 (100% over 24bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA4671031.0857021407509997RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG4000630.9298789678834587Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC3915300.9100454485803751RNA PCR Primer, Index 1 (100% over 31bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG3558230.8270505495114417No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG3057910.7107596040324917No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA2982760.6932922540310065RNA PCR Primer, Index 1 (100% over 23bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA2758170.6410900965215777RNA PCR Primer, Index 1 (100% over 22bp)
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG2257170.5246410965116761No Hit
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG2074650.48221740093920656No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG1955970.45463223662548363No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA1951990.45370715275314955No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG1872200.43516131301105365No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA1774110.4123619469212907RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC1505070.3498281366165722No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1464910.3404936219650799RNA PCR Primer, Index 1 (100% over 25bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1369800.3183869066138988No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGGAATTCTCGGG1349180.31359413539592634No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1266090.2942812663124479RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGATGGAATTCTC1253640.2913874737972318No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1237830.2877127059526079RNA PCR Primer, Index 1 (100% over 23bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG1234830.287015406551351No Hit
TACATCTTTTTTAATCGCGATGGACTTCTCGGGTGCCAAGGAACTCCAGTC1162700.2702500046137977RNA PCR Primer, Index 1 (96% over 31bp)
CGGGTTCGATTCCCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAAC1121920.26077138141937894RNA PCR Primer, Index 1 (100% over 24bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC1103720.2565410983850871No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGTGGAATTCTCG1099430.2555439602412898No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA1046710.24329008542986857RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1036160.2408379158687818No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG1007720.23422751754486648Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC973110.22618300678569936RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG952670.2214320735318024RNA PCR Primer, Index 12 (100% over 37bp)
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG904140.21015209355080333No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG885380.20579164796161023No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG881200.20482007746252562No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG853950.19848627456777548No Hit
TCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGGG816010.18966776147321326No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC811870.18870548829947875No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG803370.1867298066625842No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC792350.18416839352863387RNA PCR Primer, Index 1 (100% over 26bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT762450.17721864282944014No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG762230.17716750754001465No Hit
ATCCCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGG761680.17703966931645088No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAATGGAATTCT700200.16274968025335956No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT697840.16220113805770411RNA PCR Primer, Index 12 (100% over 36bp)
GCCTGTGTAGCTCAACCGGATAGAGCATATGGAATTCTCGGGTGCCAAGGA694940.16152708196982246RNA PCR Primer, Index 1 (100% over 22bp)
CAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGC665210.15461684490336663No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC662650.15402181608096072No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG649340.15092813107071765No Hit
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG647250.15044234582117536Illumina Small RNA Adapter 2 (100% over 21bp)
TGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGTGC633580.14726498488278142No Hit
CAGGTTCCAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC632110.14692330817616553RNA PCR Primer, Index 1 (100% over 24bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG625960.1454938444035889No Hit
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA618470.14375292023178418RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCATGGGCTTGTCCCGCATCGGTTCAAATCCGGTTGGTGTCGCCATGG612840.14244432168875873No Hit
GCCCCAATAGTATATCGGTTAGAACACATGGAATTCTCGGGTGCCAAGGAA603630.14030361252690007RNA PCR Primer, Index 1 (100% over 23bp)
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC587750.13661257436291357RNA PCR Primer, Index 1 (100% over 33bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT585590.1361105187940086RNA PCR Primer, Index 1 (97% over 35bp)
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA564020.1310969360989715RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG559140.12996266240626028No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT559120.12995801374358523RNA PCR Primer, Index 1 (100% over 30bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT551180.128112494661592No Hit
CGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGATGGAATTCTCGGGT547750.1273152490128216No Hit
CCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG520320.12093960815399607No Hit
ATCCTAAGGTCGTGAGTCCGAGTCTCACTGAAAGCACCATGGAATTCTCGG515170.11974257751517173No Hit
TCCGATATCGTTCAGCGGTTAGGATATTGGAATTCTCGGGTGCCAAGGAAC509540.11843397897214629RNA PCR Primer, Index 1 (100% over 24bp)
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT506990.11784127448107792RNA PCR Primer, Index 1 (100% over 25bp)
GTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAG498500.11586791717552092No Hit
CACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCG475660.1105591444006184No Hit
CCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAAC472890.10991530462012454RNA PCR Primer, Index 1 (100% over 24bp)
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC471680.10963406052828427RNA PCR Primer, Index 2 (100% over 34bp)
CCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTG465470.10819065076768247No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC462390.10747475671572539RNA PCR Primer, Index 1 (100% over 26bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG456200.10603599561779867No Hit
CCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT453970.10551766972953104RNA PCR Primer, Index 12 (100% over 36bp)
CGGGGTCACTGGTTCGAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC450080.10461350483923461No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACCATGGAATTCTCGGGTGCCAAG448120.1041579358970801No Hit
TGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGTG434540.10100149394072389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC11547150.045.0052761
GCCCCAA1795450.044.9552231
CACCGCT11475200.044.9463772
CCCCAAT1784050.044.935292
TCCGATA1006100.044.9278831
CCGACCA516100.044.882821
TCCGACA2090450.044.8762171
ACCGCTG11514400.044.863183
CCCAATA1789200.044.8580473
CCAATAG1731900.044.816974
GACCATA515500.044.7514153
GCTGGTC11550750.044.7425586
AATAGTA1735200.044.7409826
ATAGTAT1734850.044.7360737
CAATAGT1736300.044.711125
GTATATC1750050.044.6997710
TATATCG1807050.044.68797311
CCGCTGG11574100.044.6733634
CCGACAT2083550.044.6674582
GTCTAAT11669700.044.66127810
Produced by FastQC (version 0.11.2)