FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l02n01_sd2_2_smallrna.351000000408cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l02n01_sd2_2_smallrna.351000000408cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38394768
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG696548818.14176348194108Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGATGGAATT28044047.304130604461524No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA14346893.7366783932644156RNA PCR Primer, Index 1 (100% over 22bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG10046462.6166221397665432Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG10012382.6077459303830146No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA8337182.171436483220839RNA PCR Primer, Index 1 (100% over 23bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG4015141.0457518586907466Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG3617370.9421518056835243No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA3572550.9304783401738487RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC3442400.8965804924254263RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG3208810.8357414739424914No Hit
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2907230.7571943135585557RNA PCR Primer, Index 1 (100% over 31bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA2698060.7027155366585364RNA PCR Primer, Index 1 (100% over 23bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA2376200.6188864066062334RNA PCR Primer, Index 1 (100% over 22bp)
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA2012330.5241156815949506RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG1966570.5121973910611988No Hit
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG1952690.5085823151737757No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG1813710.4723846749119568No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGGAATTCTCGGG1701710.4432140337454311No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA1538070.400593643383911No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC1468430.3824557554300107No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG1436100.37403533731470917No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGTGGAATTCTCG1291650.3364130237744893No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1241820.32343469297691807No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT1059220.2758761297893505RNA PCR Primer, Index 11 (100% over 37bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGATGGAATTCTC1057160.27533959835360905No Hit
CGGGTTCGATTCCCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAAC1014380.2641974552366093RNA PCR Primer, Index 1 (100% over 24bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1011250.2633822399968662RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA980060.2552587373362954RNA PCR Primer, Index 1 (100% over 23bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC977630.25462583860384314RNA PCR Primer, Index 1 (100% over 24bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG936140.243819678764565No Hit
TACATCTTTTTTAATCGCGATGGACTTCTCGGGTGCCAAGGAACTCCAGTC932640.24290809622811108RNA PCR Primer, Index 1 (96% over 31bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC872070.22713250930439274No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA848240.22092593449190784RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG800190.20841120852716183No Hit
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG789430.2056087433579492Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG774740.2017827012263754No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT753980.1963757145244373RNA PCR Primer, Index 1 (100% over 25bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG752060.19587564639015398Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGC718340.1870931997818036No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA717800.18695255561903643No Hit
TCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGGG713630.18586647013988988No Hit
GCCCCAATAGTATATCGGTTAGAACACATGGAATTCTCGGGTGCCAAGGAA685720.178597250542053RNA PCR Primer, Index 1 (100% over 23bp)
TGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGTGC661370.17225524060986644No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG657210.17117175965225262No Hit
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA653520.1702106912066769RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGG645830.168207814148011No Hit
CGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGATGGAATTCTCGGGT638210.16622316874007417No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC617230.16075888256441606No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG610520.15901124861595725No Hit
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT596910.15546649480991784No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC595870.1551956245705144No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC591680.1541043300483024RNA PCR Primer, Index 6 (97% over 36bp)
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC585870.1525911030377889RNA PCR Primer, Index 1 (100% over 33bp)
GCCTGTGTAGCTCAACCGGATAGAGCATATGGAATTCTCGGGTGCCAAGGA568900.14817122999675372RNA PCR Primer, Index 1 (100% over 22bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG560170.14589748269868436No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG559700.14577507018664626No Hit
GTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAG553650.14419933465934734No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG539520.14051914573360622No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT516290.13446884221308486RNA PCR Primer, Index 1 (100% over 25bp)
CCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAAC515250.1341979719736814RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCAACTGGAATTCTCGGGTGCCAAGG502090.13077042163661465Illumina Small RNA Adapter 2 (100% over 21bp)
CAGGTTCCAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC502070.1307652125935492RNA PCR Primer, Index 1 (100% over 24bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG493520.1285383466830689RNA PCR Primer, Index 2 (97% over 35bp)
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC490250.12768666814186766RNA PCR Primer, Index 1 (100% over 26bp)
TCCGATATCGTTCAGCGGTTAGGATATTGGAATTCTCGGGTGCCAAGGAAC489110.12738975268713695RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCATGGAATTCTCGGGTGCCAAG488260.1271683683568553No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAATGGAATTCT488020.1271058598400699No Hit
CGGGGTCACTGGTTCGAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC486070.1265979781411884No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT469540.12229270404759315RNA PCR Primer, Index 1 (100% over 30bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG468640.12205829710964786No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACATGGAATTCTCGGGTGCCAAGG452170.11776865014524895Illumina Small RNA Adapter 2 (100% over 21bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT450150.11724253679563841No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG438150.11411711095636781RNA PCR Primer, Index 6 (100% over 34bp)
CCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTG437790.11402334818118968No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA435550.11343993535785916RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG434240.11309874303707214No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGTGGAATTC427430.11132506387328607No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG418870.10909559344127304No Hit
GGGACTTTAGCTCACCTGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG409000.10652493068847298RNA PCR Primer, Index 1 (100% over 29bp)
TCCCCATGGGCTTGTCCCGCATCGGTTCAAATCCGGTTGGTGTCGCCATGG393320.10244104092515939No Hit
CCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG389160.10135755996754559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC10481850.045.011931
GCCCCAA1864150.044.9566081
CACCGCT10388950.044.9525832
TCCGATA815050.044.9453241
CCCCAAT1849100.044.918932
CCCAATA1852350.044.9086463
TCCGACA1664200.044.8837551
ACCGCTG10426700.044.8684653
CCAATAG1791150.044.8622064
CCGACCA500800.044.8604131
GGGTGAG137650.044.8484571
ATAGTAT1794100.044.7800337
AATAGTA1794550.044.777266
GTATATC1813200.044.7661910
GCTGGTC10456200.044.7625276
CAATAGT1796250.044.743565
ACCGCCG115100.044.736963
GACCATA500400.044.7318573
TATATCG1875800.044.7055311
CCGACAT1657000.044.6857872
Produced by FastQC (version 0.11.2)