FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l02n01_sd2_1_smallrna.35100000040854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l02n01_sd2_1_smallrna.35100000040854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39083600
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG633659116.21291539162206Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGATGGAATT35972289.203932084045482No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA14911383.815252433245658RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG9783472.5032161827467276No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG9587392.4530468022393026No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG7968922.0389421650001536Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA7750921.9831642939749663RNA PCR Primer, Index 1 (100% over 23bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG3591080.9188201700969204Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC3568890.9131425968948612RNA PCR Primer, Index 1 (100% over 24bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA3382630.8654857792015065RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC3362930.8604453018657442No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA3143670.8043450449805034RNA PCR Primer, Index 1 (100% over 22bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2737270.7003628120234574RNA PCR Primer, Index 1 (100% over 31bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA2580250.6601873931777011RNA PCR Primer, Index 1 (100% over 23bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG2221670.5684404711950792No Hit
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG2075570.5310590631364562No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA2024520.5179973185684021No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGGAATTCTCGGG1843830.4717656510659202No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG1634690.4182547155328578No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG1611710.4123750115137807No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGTGGAATTCTCG1606310.4109933578278357No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA1520850.3891274089387876RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG1504400.38491848243252924No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA1349290.34523175961272756No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGATGGAATTCTC1181040.3021830128237931No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG1091490.27927058919853853No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC1067410.2731094372063986RNA PCR Primer, Index 10 (100% over 37bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1035010.2648195150907286RNA PCR Primer, Index 1 (100% over 24bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1003400.2567317237920765No Hit
CGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGATGGAATTCTCGGGT990150.2533415550256374No Hit
CGGGTTCGATTCCCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAAC976170.24976460714980198RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT920710.23557451207155944RNA PCR Primer, Index 1 (100% over 25bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA911450.23320523186195746RNA PCR Primer, Index 1 (100% over 23bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG874350.22371275931592793No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAATGGAATTCT846200.21651024982345538No Hit
TACATCTTTTTTAATCGCGATGGACTTCTCGGGTGCCAAGGAACTCCAGTC839700.2148471481644475RNA PCR Primer, Index 1 (96% over 31bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC826720.2115260620823056RNA PCR Primer, Index 1 (100% over 24bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC822780.21051796661515315No Hit
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG781460.19994575729973696No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG745340.19070402931152708Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG729020.18652836483844887Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG709590.1815569701869838No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA697710.1785173320779048RNA PCR Primer, Index 1 (100% over 22bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG672600.17209264243826056No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG670880.1716525601531077No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG650150.16634854516984104No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATATGGAATTCTCGGGTGCCAAGGA646000.16528671872601297RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCATGGGCTTGTCCCGCATCGGTTCAAATCCGGTTGGTGTCGCCATGG631280.16152043312284436No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC620880.15885947046843177No Hit
GCCCCAATAGTATATCGGTTAGAACACATGGAATTCTCGGGTGCCAAGGAA614320.1571810171018023RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAATGGAAT607740.15549744649929895No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG592600.15162369894277905No Hit
TGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGTGC592470.1515904369095989No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG591600.15136783714908555RNA PCR Primer, Index 7 (97% over 36bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC591500.1513422509697162No Hit
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC582600.14906508100584387RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGAA571900.14632735981332323No Hit
CAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGC571240.1461584910294855No Hit
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA558720.14295510137244266RNA PCR Primer, Index 1 (100% over 22bp)
TCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGGG551200.1410310206838674No Hit
ATCCCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGG550230.1407828347439847No Hit
CGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGAAGCATCTCATGGAA533180.1364203911615102No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC511120.1307760799926312RNA PCR Primer, Index 1 (100% over 26bp)
CAGGTTCCAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC509540.13037181835859543RNA PCR Primer, Index 1 (100% over 24bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA505940.12945071590129875RNA PCR Primer, Index 7 (97% over 35bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGACTCTTAATCTTGGAATTCT499840.1278899589597683No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG496180.12695350479485001No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGTGGAATTC491520.1257611888362382No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT473310.12110194557307925RNA PCR Primer, Index 1 (100% over 25bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT471380.12060813231125075No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT439060.11233867913907625RNA PCR Primer, Index 3 (100% over 34bp)
GTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAG438920.11230285848795915No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG431110.11030457787921276No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA426170.10904062061836678RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG417570.10684020919260251RNA PCR Primer, Index 7 (97% over 36bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT412900.10564533461605378No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG408080.10441208077045104No Hit
ATCCTAAGGTCGTGAGTCCGAGTCTCACTGAAAGCACCATGGAATTCTCGG400480.1024675311383803No Hit
GGGACTTTAGCTCACCTGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG394730.10099632582464256RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC9948700.045.0048031
TAAGCTA351.2141209E-744.996198
CTAGGCC1600.044.99618511
CCGACCA567050.044.9476131
CACCGCT9862500.044.9463732
CCCCAAT1725900.044.926192
GCCCCAA1741150.044.913671
TCCGATA740000.044.9099431
CCCAATA1731000.044.861973
ACCGCTG9895150.044.861213
CCAATAG1672300.044.835284
TCCGACA1360050.044.8299671
GGGTGAG128150.044.783271
GACCATA567000.044.76413
ATAGTAT1675800.044.747827
AATAGTA1675950.044.74586
GCTGGTC9926850.044.743086
CAATAGT1677050.044.7190975
GTATATC1692600.044.7157310
TATATCG1751250.044.68529511
Produced by FastQC (version 0.11.2)