FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l01n01_nycb20_3_smallrna.351000000407b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l01n01_nycb20_3_smallrna.351000000407b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24381807
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG382475315.686913607346659Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGATGGAATT13296815.453578563721713No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG12888845.286252983628326Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA10689784.384326395496445RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA5929452.4319157312663497RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC5597982.2959660044885104RNA PCR Primer, Index 1 (100% over 24bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG4676621.9180776880072918No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG3925101.6098478673053231No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA3026001.2410893089261186RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC2629831.0786034029389209No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG2353690.9653468260166279Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG2037300.8355820386897493No Hit
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1945790.7980499558543794RNA PCR Primer, Index 1 (100% over 31bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG1849550.7585779019577998Illumina Small RNA Adapter 2 (100% over 21bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA1685420.6912613162756969RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA1569860.6438653213849163RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC1560890.6401863487804657RNA PCR Primer, Index 3 (100% over 51bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG1532150.6283988713387814No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1492900.6123008028076016RNA PCR Primer, Index 1 (100% over 25bp)
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG1418440.5817616389138015No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1263940.518394719472597No Hit
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG1165970.47821312013502526No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA1069140.4384990825331363RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGATGGAATTCTC1042130.4274211505324441No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1003380.41152815293796724RNA PCR Primer, Index 1 (100% over 24bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG958280.39303075444736313No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC948910.3891877250935503No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG938960.385106813453162Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT924100.379012105214351No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC815280.33438046655032583RNA PCR Primer, Index 1 (100% over 26bp)
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG752890.30879171506853453No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG721090.2957492034942283No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCTGGAA695580.28528648430364495No Hit
CCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA685670.2812219783381929RNA PCR Primer, Index 3 (100% over 36bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC665170.27281406993337287RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA637420.2614326329463604RNA PCR Primer, Index 1 (100% over 23bp)
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT628320.25770034189836705RNA PCR Primer, Index 1 (100% over 25bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGGAATTCTCGGG619780.2541977302994811No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAATGGAATTCT610190.2502644697335189No Hit
CCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT601160.24656088861666406RNA PCR Primer, Index 1 (97% over 35bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG594460.24381293806484483No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG586880.24070406266442843No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCTGGAAT582840.23904708949586878No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA573530.23522866865446027RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGTGGAATTCTCG543050.22272754435304978No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG528150.21661643043930254No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG520670.21354856922622673No Hit
GCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGA488610.20039942076483502RNA PCR Primer, Index 1 (100% over 22bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT472910.1939601933523631RNA PCR Primer, Index 1 (97% over 35bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG472190.19366489120351088No Hit
CAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGC461520.1892886774142704No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC460290.1887842029099812No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA459130.1883084383368304RNA PCR Primer, Index 3 (100% over 36bp)
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA449690.18443669905187915RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG448810.184075774203282RNA PCR Primer, Index 3 (100% over 37bp)
TGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGTG441950.1812622009517178No Hit
CCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG434710.17829277378825942No Hit
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG422710.17337107130738916Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG422260.17318650746435651RNA PCR Primer, Index 3 (100% over 38bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG420180.17233341236767233No Hit
GCCCCAATAGTATATCGGTTAGAACACATGGAATTCTCGGGTGCCAAGGAA413670.1696633887718002RNA PCR Primer, Index 1 (100% over 23bp)
TCCCCATGGGCTTGTCCCGCATCGGTTCAAATCCGGTTGGTGTCGCCATGG412940.16936398520421395No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG411060.16859291848221092RNA PCR Primer, Index 3 (100% over 37bp)
CACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTCG407870.16728456590604626No Hit
CGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG407220.16701797368833246RNA PCR Primer, Index 3 (100% over 38bp)
ATCCCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGG403290.16540611612584744No Hit
ACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTC397050.1628468308357949No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC392120.16082483139990403RNA PCR Primer, Index 1 (100% over 26bp)
TTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATT391150.16042699378270034No Hit
TCCCCATGGGCTTGTCCCGCATCAGTTCAAATCTGGTTCGTGTCGCCATGG390140.16001275049056043No Hit
CACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCG385280.158019460985808No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGTGGAATTCTCGGGTGCCAAGG381770.15657986301015342Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGTTCGATTCCCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAAC373340.15312236701734205RNA PCR Primer, Index 1 (100% over 24bp)
GGGGTAGTAGCTCACCGGTAGAGCAAGGGATGGAATTCTCGGGTGCCAAGG366560.15034160511565037Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTG353840.14512460048592787No Hit
CGGGGTCACTGGTTCGAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC345710.1417901470551383No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGTGGAATTCTCGGGTGCCAAGG342290.14038746184809026Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACT336160.13787329216411237RNA PCR Primer, Index 1 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATTGGAATTCTCGGGTGCCAAGGAAC334530.13720476091046083RNA PCR Primer, Index 1 (100% over 24bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA332800.1364952154694687No Hit
GGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAAC325870.1336529322867661RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGAA311590.12779610633453048No Hit
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC308720.12661899915785568No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA301440.12363316631946106RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC294190.12065963773726862No Hit
AGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGG291830.11969170291603079Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTC286350.1174441254497667No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGCATCCCATGGTCGTCGCCATGGA285890.11725546018800002No Hit
GGTCCCGTAGTGTAGCGGTTATCACTTGGTGGAATTCTCGGGTGCCAAGGA284400.11664434879662527RNA PCR Primer, Index 1 (100% over 22bp)
ACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTCGG284350.116623841702955No Hit
CGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGATGGAATTCTCGGGT278210.11410557060024304No Hit
GCACCGCTGGTCTAATGGTAGAATTGGAATTCTCGGGTGCCAAGGAACTCC276640.11346164785899585RNA PCR Primer, Index 1 (100% over 27bp)
TCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT262560.1076868502814414RNA PCR Primer, Index 3 (100% over 34bp)
CGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGGG260720.10693218923437463No Hit
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCCTGGAATTCTCGGGT257860.10575918347643389No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT257000.10540646146530484RNA PCR Primer, Index 3 (100% over 34bp)
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA255070.10461488764963155RNA PCR Primer, Index 1 (100% over 22bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCTGGAATTCTCGGGTGCCAAGGA248950.1021048193843877RNA PCR Primer, Index 1 (100% over 22bp)
TAAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTC248470.1019079512851529No Hit
CCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAAC246470.1010876675383412RNA PCR Primer, Index 1 (100% over 24bp)
GGGACTTTAGCTCACCTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTC245120.10053397600924328RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGC551.8189894E-1245.018930
CGAGGGG207.021555E-445.018929
CCGCACA1500.045.018929
CTATCCC207.02688E-445.01197432
CGCACGA3000.045.01197432
GCACCGC6887200.044.998391
CTAGTCG1400.044.9955412
ATCAGTC551.8189894E-1244.9952666
TGCCCTA1500.044.9952628
CCCGATC207.042731E-444.9913913
CTCTAGG800.044.9911165
GCCCCAA926750.044.965511
TCCGACA1634250.044.953771
CACCGCT6828800.044.939692
GCACTGC298750.044.917291
CCGACCA331200.044.9169851
CACTGCT295400.044.9134832
CCCCAAT916150.044.9118082
TACATCT214850.044.8852581
TCCGATA661500.044.865721
Produced by FastQC (version 0.11.2)