FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l01n01_nycb20_2_smallrna.35100000040798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l01n01_nycb20_2_smallrna.35100000040798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53161085
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG1010275219.004036505274488Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGATGGAATT36749926.9129364082768445No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG26764685.034637648949414Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA20503783.856915260476719RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG13253612.493103743085755No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG12035652.2639963048158256No Hit
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC10451241.9659568648758767RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA9775991.838937260215814RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC5668201.0662310598062474No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG4890460.919932315151205Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG4635210.8719178699983269Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA4421200.8316609790789634RNA PCR Primer, Index 1 (100% over 22bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA4192390.7886200968245851RNA PCR Primer, Index 1 (100% over 22bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG3363910.6327767764709844No Hit
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG3306850.6220433612293654No Hit
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC3219250.6055651422464383RNA PCR Primer, Index 1 (100% over 31bp)
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA2857420.5375021973309988RNA PCR Primer, Index 1 (100% over 23bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG2792860.5253579756696087No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG2747210.5167708672612683No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT2666530.5015943523349834RNA PCR Primer, Index 2 (100% over 37bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGATGGAATTCTC2514210.4729418144870445No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA2510370.4722194816001216No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT2217310.4170926910163703RNA PCR Primer, Index 1 (100% over 25bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG2147880.40403238571974215Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC2120360.39885566669679523RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG2116980.398219863270285No Hit
CAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGC2073130.38997134840268965No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC1971780.3709066509835155No Hit
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG1958880.3684800639415091No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG1906860.3586947106139764No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC1728720.3251852365315719RNA PCR Primer, Index 1 (100% over 26bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA1681240.31625389135680737RNA PCR Primer, Index 1 (100% over 23bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT1619800.30469656516604204No Hit
GTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAG1601290.30121469492204683No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1546770.2909590727879237RNA PCR Primer, Index 1 (100% over 24bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA1540000.28968558485967694RNA PCR Primer, Index 2 (100% over 36bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1508370.28373574391869544RNA PCR Primer, Index 2 (100% over 35bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAATGGAATTCT1456670.2740105849984063No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGGAATTCTCGGG1388540.2611948194812051No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCTGGAA1319220.2481552060120669No Hit
TCCCCATGGGCTTGTCCCGCATCAGTTCAAATCTGGTTCGTGTCGCCATGG1303760.24524706371211197No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT1249500.23504034953387426RNA PCR Primer, Index 1 (100% over 25bp)
CGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACT1245310.23425217901402876RNA PCR Primer, Index 1 (100% over 25bp)
CCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAAC1228140.23102237285036603RNA PCR Primer, Index 1 (100% over 24bp)
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC1218090.22913189224787267RNA PCR Primer, Index 1 (100% over 26bp)
CGGGTTCGATTCCCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAAC1078980.20296425477395733RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG1070980.20145939459286807No Hit
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG1045680.19670027427017336Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1037020.19507126312414427RNA PCR Primer, Index 2 (100% over 35bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGTGGAATTCTCG1032310.194185276692528No Hit
TCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGG1020440.19195244039883685Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCATGGGCTTGTCCCGCATCGGTTCAAATCCGGTTGGTGTCGCCATGG1006540.1893377458341943No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG1005780.18919478411699084No Hit
GCCCCAATAGTATATCGGTTAGAACACATGGAATTCTCGGGTGCCAAGGAA987640.185782513656371RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA959550.18049857334552144RNA PCR Primer, Index 2 (100% over 36bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA940380.1768925521365864RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGTCACTGGTTCGAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC934510.17578836097871217No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG921190.1732827687771986No Hit
TGTAAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTC880420.1656136250793226No Hit
AAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCC854380.16071530518987714No Hit
CCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG843660.15869879254721755No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG841160.15822852374062718No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA836190.15729362935312552RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCTGGAAT808120.15201345119272866No Hit
CACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCG800580.15059512047205206No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC799850.15045780198052766RNA PCR Primer, Index 2 (100% over 34bp)
CCGGGCGGTGCACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT795790.1496940854386249RNA PCR Primer, Index 2 (100% over 37bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGTGGAATTCTCGGGTGCCAAGG792530.14908085491483103Illumina Small RNA Adapter 2 (100% over 21bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG790300.1486613751393524No Hit
TAAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTC750470.1411690525127544No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA728770.13708711927154985RNA PCR Primer, Index 1 (100% over 22bp)
GGGGTAGTAGCTCACCGGTAGAGCAAGGGATGGAATTCTCGGGTGCCAAGG726400.13664130444290218Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA719270.1353000978065064RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGA693900.13052780995722715RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTGGAATTCTCGGGTGCCAAGGAACTCC662150.12455539611352928RNA PCR Primer, Index 1 (100% over 27bp)
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGACCTCCAGTCACCGAT660270.1242017539709733RNA PCR Primer, Index 2 (97% over 37bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG657310.12364495570397029No Hit
AGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCA650950.12244859186000437No Hit
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT635390.11952163880778581RNA PCR Primer, Index 2 (100% over 37bp)
TCCGATATCGTTCAGCGGTTAGGATATTGGAATTCTCGGGTGCCAAGGAAC633240.11911720763411807RNA PCR Primer, Index 1 (100% over 24bp)
TGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGTG629440.11840239904810068No Hit
GGTCCCGTAGTGTAGCGGTTATCACTTGGTGGAATTCTCGGGTGCCAAGGA616330.11593630942634073RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTG598540.11258987659864354No Hit
TTGGGGTTGCGGGTTCGAGTCCCGCCACTGGCTCTGGAATTCTCGGGTGCC595240.11196912177394423No Hit
ATCCCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGG593020.11155152307369197No Hit
ACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTC585720.11017833815844805No Hit
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC584810.11000716031284914RNA PCR Primer, Index 1 (100% over 33bp)
GGGACTTTAGCTCACCTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTC580750.10924344377094637RNA PCR Primer, Index 1 (100% over 26bp)
CGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG577930.1087129805571124RNA PCR Primer, Index 2 (100% over 38bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGCATCCCATGGTCGTCGCCATGGA577550.10864149969851067No Hit
CACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTCG571250.1074564223059029No Hit
TTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATT570250.10726831478326673No Hit
CGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGGG567910.10682814318029815No Hit
GCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGA561620.10564494686291674RNA PCR Primer, Index 1 (100% over 22bp)
GGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAAC557970.10495835440529479RNA PCR Primer, Index 1 (100% over 24bp)
GGGACTTTAGCTCACCTGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT555850.10455956645730613RNA PCR Primer, Index 1 (100% over 30bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA554840.10436957785944362No Hit
TCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGAA544660.10245464327900755No Hit
AAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCG544150.10235870844246311No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGTGGAATTCTCGGGTGCCAAGG540400.10165330523257754Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAATGGAAT539560.10149529491356318No Hit
GGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAA537230.10105700438582095No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGTGGAATTC534340.10051337364540246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC16011100.044.9944041
ACTAGCG207.042642E-444.99160413
CGCGCTC302.1701508E-644.991613
CCTTAGC351.2151577E-744.9913065
CCGACCA817450.044.9657941
TCCGACA3324950.044.96121
GCACTGC574300.044.9547461
GCCCCAA2543150.044.948661
CCCCAAT2521850.044.919432
CACCGCT15885600.044.9168852
CACTGCT570850.044.8887632
TCCGATA875250.044.8842851
CCCAATA2530000.044.843183
CCGACAT3311400.044.8266532
GGGTGAG284650.044.811391
CCAATAG2445550.044.8076674
ACCGCCG158900.044.779363
ACCGCTG15957000.044.7763633
CAATAGT2447950.044.765245
ACGTGTG1271700.044.76290545
Produced by FastQC (version 0.11.2)