FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l01n01_nycb20_1_smallrna.35100000040755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l01n01_nycb20_1_smallrna.35100000040755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45506332
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG823753718.101957767108104Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGATGGAATT28905896.352058873916711No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG20215964.442449899060201Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA15708163.451862479269918RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG10494242.3061054448422698No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG8229551.808440636349245No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA7679831.6876398651510738RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC6366291.3989899251822802RNA PCR Primer, Index 1 (100% over 24bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA4656351.0232312285683671RNA PCR Primer, Index 1 (100% over 22bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG3917660.8609043682096812No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG3552900.7807484901222098Illumina Small RNA Adapter 2 (100% over 21bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG3393940.7458170876088189No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA3099660.6811491640328208RNA PCR Primer, Index 1 (100% over 37bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG2983030.6555197637111249Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2980140.6548846872562701RNA PCR Primer, Index 1 (100% over 31bp)
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA2790120.6131278609754792RNA PCR Primer, Index 1 (100% over 23bp)
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG2692160.5916011864019275No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG2645160.5812729533991006No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG2547800.5598781286085638No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA2457250.539979798855245RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC2185210.4801991072363292No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA2057040.4520337960879818RNA PCR Primer, Index 1 (100% over 23bp)
CAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGC2040860.448478246939349No Hit
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC1962310.43121691284632657RNA PCR Primer, Index 1 (100% over 24bp)
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG1805860.3968370819252142No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1740850.3825511579355594No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC1638020.3599543026232042No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG1507200.33120665493320794Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT1506310.3310110777550693No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGATGGAATTCTC1410210.3098931375088636No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG1354250.2975959477463488No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1324510.29106059350158126RNA PCR Primer, Index 1 (100% over 24bp)
CCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1279310.2811279098477988RNA PCR Primer, Index 1 (100% over 35bp)
TCCCCATGGGCTTGTCCCGCATCAGTTCAAATCTGGTTCGTGTCGCCATGG1262940.27753060826787795No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT1260710.27704056657433956RNA PCR Primer, Index 1 (100% over 25bp)
CGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACT1209530.2657937800831761RNA PCR Primer, Index 1 (100% over 25bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1180300.2593704981539712RNA PCR Primer, Index 1 (100% over 25bp)
GTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAG1162360.25542818964182834No Hit
CCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAAC1147970.2522659923458564RNA PCR Primer, Index 1 (100% over 24bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC1146640.2519737253268402RNA PCR Primer, Index 1 (100% over 36bp)
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC1079090.23712963725575598RNA PCR Primer, Index 1 (100% over 26bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGGAATTCTCGGG1030890.22653770468689938No Hit
TCCCCATGGGCTTGTCCCGCATCGGTTCAAATCCGGTTGGTGTCGCCATGG996790.21904424201889092No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA992310.21805976363904697RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTC980360.21543375546066862No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC977750.2148602089045542RNA PCR Primer, Index 1 (100% over 26bp)
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG967680.21264733004628897Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCTGGAA956220.21012899919070605No Hit
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT952090.2092214331842874RNA PCR Primer, Index 1 (100% over 35bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG899200.1975988748115317No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC865610.19021748445908582RNA PCR Primer, Index 1 (100% over 51bp)
GCCCCAATAGTATATCGGTTAGAACACATGGAATTCTCGGGTGCCAAGGAA835750.18365576025771535RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGTCACTGGTTCGAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC834240.18332393830379473No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGACCTCCAGTCACATCA795900.17489873716914822RNA PCR Primer, Index 1 (97% over 37bp)
CACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCG781700.17177829230446434No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA780720.1715629376588735RNA PCR Primer, Index 1 (100% over 22bp)
TCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGG771340.16950168605107527Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCTGGAAT762990.1676667765707858No Hit
ATCCCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGG758990.16678777801735373No Hit
TAAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTC758220.16661857079581804No Hit
CCGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTG757530.166466943545351No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA755770.1660801841818409RNA PCR Primer, Index 1 (100% over 22bp)
AAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCC754200.16573517724961878No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG718610.15791428762045687No Hit
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA718600.15791209012407328RNA PCR Primer, Index 1 (100% over 37bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAATGGAATTCT718060.15779342531935997No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGTGGAATTCTCGGGTGCCAAGG715760.1572880011511365Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG703960.1546949554185119No Hit
CGGGTTCGATTCCCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAAC678430.14908474715123163RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG671350.14752891971165683No Hit
TCCGATATCGTTCAGCGGTTAGGATATTGGAATTCTCGGGTGCCAAGGAAC666340.14642797402348315RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG658420.14468755688768764No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTC649720.14277573503397287RNA PCR Primer, Index 1 (100% over 51bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGTGGAATTCTCG648030.1424043581451478No Hit
TGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGTG638760.14036727899756896No Hit
CCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC631320.13873234168818527RNA PCR Primer, Index 1 (100% over 36bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA628870.13819395507420812No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGTGGAATTCTCGGGTGCCAAGG620360.13632388565178138Illumina Small RNA Adapter 2 (100% over 21bp)
TTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATT613840.1348911180096871No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA610410.13413737675011908RNA PCR Primer, Index 1 (100% over 34bp)
GGGACTTTAGCTCACCTGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT609020.13383192475280142RNA PCR Primer, Index 1 (100% over 30bp)
GGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAAC591090.12989181373704212RNA PCR Primer, Index 1 (100% over 24bp)
TCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGGG566030.12438488779979015No Hit
AGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCA559360.12291915771194216No Hit
TCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA559240.12289278775533918RNA PCR Primer, Index 1 (100% over 34bp)
ACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTC558870.12281148038914673No Hit
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA555230.12201159170552353RNA PCR Primer, Index 1 (100% over 22bp)
GGGGTAGTAGCTCACCGGTAGAGCAAGGGATGGAATTCTCGGGTGCCAAGG539840.1186296447711936Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG536670.11793303841759867No Hit
TTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC529050.11625854617331055RNA PCR Primer, Index 1 (100% over 38bp)
CGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGA522080.11472689119395516RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC519080.11406764227888111No Hit
CGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGGG515720.11332928349399815No Hit
TTGGGGTTGCGGGTTCGAGTCCCGCCACTGGCTCTGGAATTCTCGGGTGCC509170.11188992336275312No Hit
CACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAATTCTCG507640.11155370641606535No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG506900.11139109168368043No Hit
TTCTTTTGATCCGGGTTCGATTCCCGGTGACCGAGCCATGGAATTCTCGGG497290.10927929765905985No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACATGGAATTCTCGGGTGCCAAGG482700.10607315043541633Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCG478660.10518536189644992No Hit
GCACCGCTGGTCTAATGGTAGAATTGGAATTCTCGGGTGCCAAGGAACTCC469480.1031680602163233RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG468760.10300984047670553No Hit
GGGACTTTAGCTCACCTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTC463480.1018495623861752RNA PCR Primer, Index 1 (100% over 26bp)
CGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC461980.10151993792863814RNA PCR Primer, Index 1 (100% over 38bp)
CTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCT457570.10055084202347928RNA PCR Primer, Index 1 (100% over 51bp)
GGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAA456510.10031790740681978No Hit
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCCTGGAATTCTCGGGT455830.10016847765273633No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA207.020383E-445.0205126
CTCACGC406.7975634E-945.0205130
GCACCGC12619000.044.998451
CCGACCA596300.044.9515881
TCCGACA2671500.044.9435231
CACCGCT12488650.044.9420742
CCCCAAT1770450.044.930622
GCCCCAA1790700.044.925271
GCACTGC360450.044.9198651
TCCGATA957750.044.8950651
CCCAATA1776450.044.8487433
ACCGCCG148800.044.8394663
ACCGCTG12539150.044.821693
CCAATAG1714200.044.8133664
CACTGCT357650.044.800562
GGGTGAG277050.044.7890241
GACTTTA477250.044.7889183
CCGACAT2659350.044.7851642
CAATAGT1716300.044.7582475
TACATCT331300.044.7377661
Produced by FastQC (version 0.11.2)