FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah8530b-.3510000003d0da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah8530b-.3510000003d0da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10877947
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC374720.3444767656985275TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG49500.037.54157311
ACGTCTG50650.036.4711315
ACACGTC52100.035.79829813
CACACGT52700.035.3475512
AGCACAC53550.034.66028210
CACGTCT54300.034.18311714
GATCGGA54900.033.945271
CGTCTGA55650.033.19429816
CGTATGC54150.032.9074544
CAGTCAC55150.032.8441327
GAACTCC56750.032.4314921
GTATGCC55200.032.3630145
ACTGACC56700.032.1839432
GAGCACA58500.031.8428579
TCGGAAG60200.031.2497963
TCGTATG58700.030.50917843
TCCAGTC59950.030.36189325
CTCGTAT59250.030.2643642
AGAGCAC62250.030.0330378
CCAGTCA61000.029.73121526
Produced by FastQC (version 0.11.2)