FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah8530a-.3510000003d060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah8530a-.3510000003d060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366730
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1792331.4493160277615829TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG208650.042.7096911
GATCGGA214900.042.114421
ACACGTC213550.041.6355613
CACACGT214350.041.54246512
ACGTCTG215650.041.2632415
AGCACAC217200.040.95593310
CGTCTGA217700.040.8126716
CACGTCT218550.040.77599314
GAGCACA218600.040.724519
GAACTCC218950.040.3126621
CAGTCAC216400.040.19896327
TATCTCG217500.040.14742739
AGAGCAC222050.040.13238
CCGATGT217450.040.11721833
GTATGCC218300.040.05037745
TCGGAAG225750.039.8040663
CGATGTA218850.039.69608334
CGTATGC220500.039.58955844
CTCGTAT221150.039.56492242
GTCACCG221350.039.5436529
Produced by FastQC (version 0.11.2)