FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah8511i-111215.3510000003d238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah8511i-111215.3510000003d238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13617977
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG13069489.5972257847109TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1502250.044.4703061
GCACACG1488700.044.24321711
TCGGAAG1497400.044.17553
GAGCACA1487950.044.080899
ACACGTC1494700.044.069613
AGCACAC1495150.044.067410
CACACGT1494850.044.06436512
CACGTCT1494850.044.01228714
AGAGCAC1491550.043.9971248
ATCGGAA1515750.043.988042
ACGTCTG1495650.043.97182515
CGGAAGA1503550.043.8689234
CGTCTGA1499000.043.73546616
GAACTCC1492950.043.5706621
GTCTGAA1503100.043.5486517
TCGTATG1481400.043.48311645
TCCAGTC1489950.043.45517325
ACTCCAG1495350.043.349823
CAGTCAC1487550.043.3300927
CCAGTCA1488700.043.29963726
Produced by FastQC (version 0.11.2)