FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah8511a-111215.3510000003d1a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah8511a-111215.3510000003d1a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11670540
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1461361.2521785624315585TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG175550.042.265911
ACACGTC178700.041.6481913
CACACGT178750.041.54701212
ACGTCTG180750.041.32697315
CACGTCT180250.041.3157214
GATCGGA185300.040.8630831
CGTCTGA182650.040.79852716
AGCACAC183150.040.6348710
GAGCACA183400.040.4075669
CAGTCAC184100.039.7359427
GAACTCC185150.039.73571821
TCCAGTC185400.039.58539625
AGAGCAC189000.039.2817278
CACATGT186400.039.19751731
CTCGTAT187200.039.18022544
TCACATG186750.039.17208530
AGTCACA187800.039.0968528
GTCACAT187900.039.0640729
CCAGTCA187700.038.90189426
TCGGAAG193800.038.8874633
Produced by FastQC (version 0.11.2)