FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah8428i-111215.3510000003d153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah8428i-111215.3510000003d153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14776287
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG731750.49521913048927657TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG91600.040.01622432
CGTCCGC93250.039.0174133
GCACACG97350.038.8943611
AGTCACG95800.038.61430728
GATCGGA100900.038.3266831
CACACGT99650.037.9740712
ACGTCTG100550.037.92825315
CCGCACA94550.037.9083936
ACACGTC100450.037.89663713
GTCACGT99950.037.12344729
CACGTCC98900.037.1080531
GTCCGCA98700.036.90817334
AGCACAC102850.036.79257210
CACGTCT103500.036.7372714
TCACGTC101050.036.47439230
CGTCTGA104550.036.41258616
GAGCACA104250.036.2984779
TCCGCAC100650.035.96858235
CGCACAT100750.035.7988937
CATCTCG103250.035.4965141
Produced by FastQC (version 0.11.2)