FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah8428a-111215.3510000003cf64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah8428a-111215.3510000003cf64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16783423
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG204690.12195962647190622TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38550.028.88819111
AGTCACG39900.026.5622728
ACACGTC42150.026.31498313
GATCGGA45550.025.150251
CACACGT44950.025.12550712
GTCACGT42500.024.88419229
ACGTCTG46000.024.26099815
CGATCTC42850.024.10013640
AGCACAC48250.023.64014810
GATCTCG44450.023.5869541
CACGTGA45350.023.4195931
CGTCTGA48750.023.26166216
TCACGTG46000.023.03974330
CACGTCT48650.022.84610214
GAGCACA51750.021.8673369
CAGTCAC50700.021.12594827
GAACTCC53700.020.65585721
TCCAGTC54800.020.20035425
CTCGTAT53900.020.0778244
AGAGCAC57750.019.517498
Produced by FastQC (version 0.11.2)