FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah7660i-111215.3510000003d394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah7660i-111215.3510000003d394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6261238
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC269520.43045800207562784TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42450.040.17168411
ACACGTC43200.039.4761513
ACGTCTG43500.039.10326815
CACACGT43950.039.05657612
GATCGGA45150.038.483571
CACGTCT44450.038.41847214
CATCTCG40650.038.02504739
CAGTCAC44050.037.9035727
AGCACAC45000.037.89528310
GAGCACA45000.037.5453269
GAACTCC45500.037.18367421
CGTCTGA45900.037.10767416
AGTCACC45150.036.88102728
CCAGTCA45700.036.83049426
TCCAGTC46250.036.58273725
GTATGCC44700.035.6354345
TCGGAAG48300.035.547293
AGAGCAC47700.035.5144468
ACTCCAG48350.034.9453423
CTCGTAT45250.034.90424342
Produced by FastQC (version 0.11.2)