FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah7660a-111215.3510000003d337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah7660a-111215.3510000003d337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13394632
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3499942.6129422592572906TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG410600.043.26867711
GATCGGA417100.043.215031
GTCACGC406750.042.9667429
ACACGTC414100.042.87804413
CACACGT414300.042.84967812
CACGTCT415550.042.6970614
TCACGCC407250.042.67640330
AGCACAC417100.042.5997810
ACGTCTG416700.042.5854215
AGTCACG411150.042.56743628
CGTCTGA419200.042.28314216
CACGCCA408550.042.27624531
GAGCACA420250.042.275129
TCGGAAG424600.042.130433
AGAGCAC421700.042.1084148
GTATGCC416800.042.07238845
TATCTCG417550.041.92153539
GAACTCC416700.041.8334921
CAGTCAC418900.041.72081827
ATCGGAA433150.041.572392