FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah7011i-111215.3510000003d2f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah7011i-111215.3510000003d2f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12669310
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC12603429.947992432105616TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1422500.044.5237121
GCACACG1410950.044.41602311
ACACGTC1409200.044.3620713
CACACGT1410600.044.3393212
ACGTCTG1413350.044.2959915
CACGTCT1413200.044.278614
AGCACAC1415500.044.27466610
GAGCACA1411000.044.2402959
TCGGAAG1421300.044.2114143
AGAGCAC1415900.044.125338
CGTCTGA1418100.044.12223416
GTATGCC1401500.044.10172345
ATCGGAA1436950.044.0074232
GAACTCC1418750.043.85640721
GTCTGAA1426500.043.82123617
CAGTCAC1409800.043.80931527
CGGAAGA1432100.043.8069464
TCCAGTC1414350.043.78320725
CCAGTCA1411200.043.72422426
AGTCACC1412800.043.66230828
Produced by FastQC (version 0.11.2)