FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah7011a-111215.3510000003d2cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah7011a-111215.3510000003d2cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12425687
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG4118063.3141507588272585TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA488800.043.680881
GCACACG481400.043.5883911
ACACGTC485150.043.16011413
CACACGT486650.043.0950412
AGCACAC488700.042.9695110
GAGCACA488550.042.9136289
ACGTCTG489000.042.80891415
CACGTCT488650.042.80119314
CGTCTGA490700.042.61475416
TCGGAAG496850.042.543463
AGAGCAC493500.042.4923028
GAACTCC490650.042.07289521
TCCAGTC488900.042.06719625
ATCGGAA507850.041.9174652
CACAGTC489050.041.84160231
GTCACAG492350.041.7759529
CAGTCAC491300.041.76481227
GTCTGAA501150.041.73961617
CCAGTCA491050.041.73567226
AGTCACA492950.041.58883728
Produced by FastQC (version 0.11.2)