FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah119b-.3510000003d010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah119b-.3510000003d010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13303855
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1577981.1861073350543883TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG181100.042.9259335
GCACACG190250.042.54721511
GTCCCGA179850.042.4486236
ACCCGTC187650.042.06514432
ACACGTC193400.041.9259113
CCCGTCC188000.041.75832433
TCACCCG189200.041.7441630
CACCCGT189450.041.64172431
CACACGT194650.041.56233212
ACGTCTG196100.041.3285115
CACGTCT196500.041.26588414
CCCGATC185600.041.19368438
AGCACAC196850.041.16640510
GATCGGA200150.040.957291
CCGATCT187000.040.8835939
CCGTCCC191650.040.72821834
CGATCTC188950.040.60333640
TCCCGAT189100.040.53878837
CGTCTGA200650.040.44739516
GAGCACA200750.040.1984029
Produced by FastQC (version 0.11.2)