FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l08n01_ah119a-.3510000003cfa8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l08n01_ah119a-.3510000003cfa8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19434537
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC960270.49410490201027174TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG125850.038.93562711
GATCGGA133000.037.5014761
ACGTCTG131600.037.32296415
ACACGTC131400.037.27473413
CACACGT132050.037.09048512
CACGTCT133050.036.9152614
GTCACAC129150.036.8317529
AGCACAC135700.036.1591610
CGTCTGA135250.036.09946416
GATCTCG132700.035.79205339
CACACAG132700.035.72772631
GTATGCC138250.034.72870345
GAGCACA141100.034.7273989
CAGTCAC137450.034.5915427
GAACTCC141200.034.40148521
TCCAGTC142100.033.75723625
CACAGTG140800.033.68731333
CGTATGC143200.033.59099244
AGAGCAC146150.033.435088
CCAGTCA143150.033.4027926
Produced by FastQC (version 0.11.2)