FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l07n01_sv102.3510000003cc5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l07n01_sv102.3510000003cc5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22987540
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC5712332.4849679435033063TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA658400.043.7396241
ATCGGAA659450.043.711582
ACACGTC651200.043.68910613
CGTCTGA653700.043.50613816
CGCCAAT635800.043.419433
ACGTCTG656750.043.33835215
CTCGTAT645050.043.27898442
ACGCCAA637200.043.2755132
GTCACGC645500.043.26577429
TCTCGTA646300.043.2161741
TCGGAAG666700.043.212613
TATCTCG646300.043.19801339
TCGTATG647700.043.17505343
CGTATGC648750.042.98454344
AGTCACG651150.042.9421928
CACGTCT663550.042.9272914
CGGAAGA669100.042.8844154
TCACGCC650450.042.8258230
ATCTCGT652000.042.81000540
CACGCCA647500.042.74339331