FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l07n01_sv101.3510000003cc31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l07n01_sv101.3510000003cc31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28685275
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1028240.35845568850220194TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG133100.038.30480643
CTCGTAT132900.038.2477442
TCTCGTA132900.038.2138841
CGTATGC135500.037.56044844
GATCTCG136850.036.8964939
ATCTCGT140700.035.90281340
GTATGCC159950.032.0300645
CGTCTGA175200.029.48616216
GATCGGA181550.028.833621
ACGTCTG180450.028.81532915
ATCGGAA182600.028.7866022
ACACGTC181950.028.5889913
AGTGATC176800.028.54682236
GTGATCT184050.027.6057137
TCGGAAG191400.027.4513233
CACGTCT192650.027.03625514
CGGAAGA195800.026.8107074
GCACACG207300.025.32046511
CACACGT206650.025.20413812
TGATCTC202500.025.10145838
Produced by FastQC (version 0.11.2)