FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l07n01_bgm13.3510000003ccee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l07n01_bgm13.3510000003ccee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27740629
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC295838910.66446258302218TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3296450.044.2206413
ATCGGAA3367800.044.188672
GATCGGA3370850.044.1475221
TCGGAAG3361450.044.1449553
CGGAAGA3353000.044.100854
ACGTCTG3303700.044.08796715
GCACACG3316500.044.07258611
CGTCTGA3301300.044.06958816
CACGTCT3311600.044.0145814
CACACGT3314850.044.01239812
AATCTCG3280350.043.3886839
GTATGCC3308550.043.31629645
CTCGTAT3312550.043.26311542
TCTCGTA3313950.043.24958841
GAGCACA3390900.043.2408269
TCGTATG3321000.043.2211943
CGTATGC3312700.043.2124544
GAAGAGC3411900.043.192286
ATCTCGT3308950.043.16929640
AGCACAC3401150.043.05891810
Produced by FastQC (version 0.11.2)