FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l07n01_bgm12.3510000003ccc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l07n01_bgm12.3510000003ccc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26449774
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC10383443.9257197433898683TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1188400.043.48354341
CTCGTAT1188800.043.45755442
CGTCTGA1208050.043.4500616
TCGTATG1190100.043.4144543
CGTATGC1189250.043.34372344
CATCTCG1190800.043.18904539
ATCTCGT1195650.043.18698540
ACGTCTG1217200.043.1585615
ACACGTC1223050.043.0519213
GTATGCC1200850.042.97749745
CACGTCT1230550.042.7348414
CACACGT1246600.042.27054212
GATCATC1214650.042.22140536
GCACACG1255700.042.05909311
AGTCACC1239600.041.66533728
AGATCAT1233400.041.51176535
GAACTCC1260350.041.5035821
ACCAGAT1243450.041.39581332
GTCACCA1247900.041.34478429
CCAGATC1240100.041.3402433
Produced by FastQC (version 0.11.2)