FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l07n01_bgm11.3510000003cc81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l07n01_bgm11.3510000003cc81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38663206
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1536550.3974191897071339TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT194800.039.79263742
TCGTATG195500.039.76211543
TCTCGTA195450.039.6487941
CGTATGC198150.039.21980744
AATCTCG196700.039.03026639
CGTCTGA204650.038.7111416
ACACGTC210350.037.71422613
ACGTCTG213400.037.20821815
ATCTCGT207550.037.18479540
CACGTCT231550.034.3783614
GTATGCC228650.034.16542445
ATCGGAA244850.033.24152
GCACACG242650.033.01783811
CGGAAGA247700.032.7848664
CACACGT243800.032.70533812
GATCGGA248750.032.6346781
TCGGAAG249900.032.6147773
ACCAATC248150.030.81144536
GACCAAT254850.030.07072635
ACTGACC261750.029.6234832
Produced by FastQC (version 0.11.2)