FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l07n01_bgm10.3510000003cc67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l07n01_bgm10.3510000003cc67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31778903
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1730460.5445310683002494TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA201650.041.720134
TCTCGTA204950.041.44480541
CACCGAT203750.041.43463531
CTCGTAT205050.041.39167442
CGTCTGA209250.041.322216
ACCGATG204300.041.2570132
CGTATGC204900.041.21118544
TCGTATG206050.041.20984343
TATCTCG206850.040.95462839
CCGATGT206100.040.9503733
GTCACCG207350.040.9319629
GATCGGA215250.040.7051731
ACACGTC213700.040.5026913
ACGTCTG214300.040.4744315
ATCGGAA217850.040.378252
ATCTCGT211550.040.2042240
TCACCGA216300.039.0406430
TCGGAAG225800.038.896813
CGGAAGA228050.038.3924564
CACGTCT229350.037.88632614
Produced by FastQC (version 0.11.2)