FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_12.3510000003cd9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_12.3510000003cd9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7048879
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG72250.10249856750271923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCT5050.016.9331631
GCCGGGG6050.014.8782021
GGGGGAT7600.014.5086951
CGGGCGA3200.014.0645511
GCGGGGA6400.013.0097091
GGGGGGA5500.012.2745171
CGGGCCG1300.003020845412.1171511
GCCGGGT6350.012.0490011
GGGGAAT12900.012.0366391
CGGGGGG4800.011.7204591
CGCGGGG2901.3500539E-811.6396281
GCCCGCC2351.7562033E-611.4910361
GGGGTAT5000.011.251641
TGGTACC30500.011.21323920
GCGGATC21300.011.1984982
GGGGGAC4051.2732926E-1111.1127311
GTCGGCC17250.011.0864541
GGGGCAT4601.8189894E-1210.7624391
GGTACCT13450.010.70636221
GCCGAGT8850.010.6795231
Produced by FastQC (version 0.11.2)