FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_11.3510000003cd73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_11.3510000003cd73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235139
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGT3350.013.434441
GGGGAAT12000.013.314091
CGGGGGT5050.012.9223361
CGGGCGC1250.00225224612.60089915
GGGGGCT4350.012.4152761
CGGGGGC4000.012.3764781
CGGGGGG3950.011.9634531
GGGGGAT6450.011.8618821
CGGGTAT5900.011.8234451
GTCCGCT3052.2555469E-911.80468751
GGGCGCT1350.004006909711.6680591
GGGGGAC3702.910383E-1111.5554331
GGGGCTC3702.910383E-1111.55462
AGGGGGC11350.011.49695738
GGGAGAT19200.011.1341421
GGCCGAT4450.011.1249251
TGGTACC27150.011.10540820
GGGGACT9150.011.0668951
CGCCCAT4500.011.0013141
TAACGTA16250.010.523324
Produced by FastQC (version 0.11.2)