FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_10.3510000003cd66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_10.3510000003cd66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6825131
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA78080.11440073457930698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT4050.015.558161
GGGGGAT7550.015.4992841
CGGGGGT3250.015.2333191
CGGGGGA5250.013.7165821
GCCGGGG4850.013.455861
GTACCGA22500.013.29959622
TGGTACC31750.013.18079920
GGGCGCT1403.605423E-412.8592941
TACCGAG23250.012.77352423
GGTACCG24350.012.56644421
GGGGGAC3950.012.5337431
CGCGGGG2054.82661E-612.07519151
GGGGGGT4150.011.9297081
CGCGGGC2654.8319635E-811.88878251
GTCCGCT2654.8319635E-811.88878251
GGGGCAT4550.011.8701191
ATTGGTA36600.011.741961518
GCCGAGT9000.011.5019251
TTGGTAC37850.011.23520919
TTAGGCG26100.011.03495532
Produced by FastQC (version 0.11.2)