FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_09.3510000003cd4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_09.3510000003cd4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5880794
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA62150.10568300811080952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGC2700.015.8365811
GGGGGAT6850.015.4411211
GGGGGGT3400.015.2237091
GCCGGGG3700.013.9893541
GGGGGCT5700.013.4238041
GCCCGAT3355.456968E-1212.7638111
CGCGGGG2003.6610363E-612.3775381
GGGCGCT1852.2419257E-512.1646561
CGGGGAG7500.012.00246051
CGGGGGA3950.011.96447851
GCGGGGG4350.011.8989921
CGGGGGT4000.011.8149221
CCGCGCT3254.8748916E-1011.7716451
GCCGGGT5000.011.7023981
TGGTACC25150.011.540668520
CGGGCGA2553.761179E-711.472941
CGCCCAT4151.8189894E-1211.3878771
CGGGGAT27900.011.37331
GGTACCG19850.011.33463721
GCGGGCT3804.7293724E-1111.2523071
Produced by FastQC (version 0.11.2)