FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_08.3510000003cd16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_08.3510000003cd16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7819844
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA84020.10744459863905213No Hit
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG83930.10732950682903648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGT6050.015.6223721
CGGGCGA3200.014.76802351
GGGGCCC1553.617537E-614.5184861
GGGGGAC4850.014.383781
GGGGGAT9100.013.8484031
GGGGGCT7650.013.2374441
CGGGCCG1555.8093472E-513.0666381
GGGGTAT4400.012.7861671
GGGGCAT7050.012.4488311
CGGGGGG5900.011.8239551
GCGGGGC2706.265509E-811.6685631
CGGGGGT5300.011.4641271
GGGGGAG16050.011.3569851
GCGGGGA7500.011.1018031
CGCGGGG3256.1045284E-911.0787231
GCGGATC19700.010.9656162
GCCGGGG6200.010.88886451
GGGGAAT13450.010.8753721
CGAGGGG4405.456968E-1210.73900745
GGTACCG21750.010.65485921
Produced by FastQC (version 0.11.2)