FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_07.3510000003ccfb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_07.3510000003ccfb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8563153
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT8750.016.7174761
GGGGGAC5000.014.8528361
GCGGGCT6200.013.7929541
GGGCGCT3251.8189894E-1213.1563581
GGGGGCT6000.012.7524341
GCGGGGA7850.012.3271931
GGGGCAT6550.012.0251951
CGGGGAG10500.011.7879651
CGGGGGG6200.011.615121
GCCGAGT11250.011.6022141
GCGGATC23050.010.5435112
GGGGTAT5800.010.4761371
GTCCGCT4101.9281288E-1010.428821
GCGGGAG10000.010.3519761
GCGGGTT9350.010.3495691
GGCCCAT5900.010.2985761
GCCGGGT8950.010.0577861
TGGTACC37550.010.00681520
GGCCGAT6300.010.0019081
CGCGTAT8200.09.8799341
Produced by FastQC (version 0.11.2)