FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_06.3510000003ccd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_06.3510000003ccd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6819526
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA72860.1068402701302114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGG5100.017.2076531
GCGGGCT4200.016.0730821
GGGGGGT5600.014.86761
GGGGTAT4200.014.4657731
GGGGGGA6050.013.3898071
CGCGGGG2959.640644E-1112.9674341
GGGGGAT8950.012.8225471
CGGGGGT4950.012.7285811
GGGGGGG7200.012.5012861
CCCCCGT1454.8497602E-412.415071
GGCCCGC1300.003021800712.1166311
CGGGGAG8000.012.0949941
GGGGGTT6000.012.0012351
GCGGGGC3451.05501385E-1011.7403381
GCCGGGT6400.011.6027561
GGGGGCT5700.011.4485451
GCCGAGT8700.011.3804811
GCGGGGG6550.011.3370431
CGCGGAC1400.00525663711.2511571
GCGGGGA6800.011.2511571
Produced by FastQC (version 0.11.2)