FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_05.3510000003ccb7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_05.3510000003ccb7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8776775
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA88950.10134702097296558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGG5350.014.3016541
GCGGGCT4500.014.0025331
GGGGGAT10700.013.8810171
CGGGGGG5850.013.84865951
GGGGGGT6050.013.0188851
GGGGGAC4000.012.9398411
CGGGGGT5300.012.7381531
GGGGTAT6100.012.1743351
CGGGGGA6050.011.902981
CGGGCGA4350.011.8987041
GGGGGCA6900.011.7412551
GCGGGTT9950.011.5347511
GCGGGAT9850.011.194921
GTACCGA25700.011.11815522
TGGTACC38150.010.91086320
GGGGGCT6000.010.8769681
CGGGGAG9500.010.6598231
GGTACCG27000.010.58307721
GCGGGGG6700.010.4123321
GGGGGTC4152.419256E-1010.3030681
Produced by FastQC (version 0.11.2)