FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_04.3510000003ccab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_04.3510000003ccab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8025287
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT5050.015.5956821
GGGGGGT4950.015.4561531
GGGGGAT7950.015.2846081
CGCGGGG2850.015.0015591
GGGGGCT5950.013.99305251
GTACCGA23100.013.05159722
GGGGGAC4900.012.858481
GGGGGTT6300.012.85847951
GCGGGGA8150.012.70070651
GGTACCG24300.012.49966421
TACCGAG24950.012.44424523
TGGTACC35800.012.31833620
GGGGCAT6050.011.9020641
GGCCGAT5200.011.6839071
CGGGGGA5700.011.4485581
CGGGGGG4151.8189894E-1211.3867251
GTCGGCC19100.011.07265741
GGGGGGA5300.011.0388841
GCGGATC20800.011.0344572
GGGGCCT4700.011.0117841
Produced by FastQC (version 0.11.2)