FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_03.3510000003cc9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_03.3510000003cc9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228420
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT7750.013.937491
GGGGGAC5200.013.4153931
GGGGGCT5300.012.7376821
GTCCGCT3601.8189894E-1212.50181
GGTACCT15650.012.2203521
CGGGCGA3356.366463E-1112.0912931
GGGGGGT5450.011.97421
TGGTACC34300.011.93875320
GCCGGGG6600.011.5925781
GGGGTAT5050.011.5858271
CTGGGGG3002.2277163E-811.251621
CGGGGGA8100.011.1127111
GGGGGGA4700.011.0122231
GCCCGGT5150.010.92390251
GGGGGTA3959.822543E-1110.8243441
TACCTTA17050.010.68841523
GCCCGCT2801.2577857E-610.4479321
CGGGGAG8100.010.2792581
ATGCCGT19300.010.02521824
CGGGCCA4501.0004442E-1010.001441
Produced by FastQC (version 0.11.2)