FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_02.3510000003cc74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_02.3510000003cc74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8661447
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA92620.10693363360648632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC4250.014.2956141
GCGGGGC3550.013.9451221
CGGGGGA5650.013.5412411
GGGGGCT6200.013.4288241
GGGGGGT4900.013.317721
GCGGGGA7600.013.0276791
GGGGGAT10000.012.8263431
CGCGGGG3304.9112714E-1112.2740121
GTACCGA28650.011.85809922
TACCGAG28350.011.82458823
GGGCGCT3254.8748916E-1011.7704641
CGGGGGG5600.011.6530061
GCGGGCT5800.011.639151
CGGGGGT6050.011.5301321
GGTACCG29300.011.44198921
GCGGGAT9850.011.4225161
TGGTACC40250.011.40426320
CGGGCGA3753.8198777E-1111.4011941
GCGGATC21750.011.0696772
GGCCGCT4500.011.0011511
Produced by FastQC (version 0.11.2)