FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l05n01_4tfs_01.3510000003cc4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l05n01_4tfs_01.3510000003cc4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895695
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA119090.12034526124744144No Hit
AAGGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCAC100190.10124604689210814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCG2001.5746991E-814.6273151
CGCGGGG3750.013.8021831
CGGGGGT5950.013.2373891
GGGGGAT12250.013.042881
GGGGTAT7600.012.4361781
GGGGGCT7650.012.3548971
CGGGGAG10700.012.1981921
GCCGGGT8700.012.1570961
GCGGATC25550.012.0658182
GCGGGCT4900.011.9406641
GGGGGGT7000.011.8947391
GGGGGAC5500.011.8655141
GCGGGAT10800.011.6685121
TGGTACC49600.011.61286320
GTACCGA34050.011.49740822
TACCGAG34900.011.47496723
GCCGGGG7350.011.3283231
GGTACCG34450.011.29888821
GGGGGTA4201.8189894E-1211.25178051
GGCGGAT37800.011.0136471
Produced by FastQC (version 0.11.2)