FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_19_tga1_wt.3510000003d2a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_19_tga1_wt.3510000003d2a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13450718
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG495040.36803983252046474No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG320850.23853745205274546No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC244600.18184902843104733No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG237120.17628798700560075No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA228900.1701767890754977No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT162250.12062553091961335No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA156880.11663317898717378No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC153210.11390470010597205No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT152230.11317611446467021No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT145450.10813549135443921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22750.025.8115444
GCACACG26150.024.95246311
GTATGCC24000.024.74961945
ACGTCTG27300.023.98510715
GCTGACT50950.023.4092031
AGTCACG25400.022.76590328
ACACGTC30050.021.41501413
CTCGTAT27700.021.36145442
AGCACAC31200.021.20112210
TCGTATG27950.021.00939443
GTCACGA27700.020.5505329
CACGTCT31850.020.4873314
CACACGT32350.019.82246612
GACTCTA65200.018.5682164
CGTCTGA35850.018.3903416
CTGACTC67100.017.9082972
ACTCTAG66900.017.8262945
TCACGAT32900.017.71288730
CAGTCAC34250.017.40868427
GAGCACA37600.017.292789
Produced by FastQC (version 0.11.2)