FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_18_tga1_wt.3510000003d27b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_18_tga1_wt.3510000003d27b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14661084
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC2470271.6849163404288523TruSeq Adapter, Index 8 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG486530.33185131467768686No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG366920.25026798836975495No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA289240.19728418444366053No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC255060.1739707650539346No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG232530.15860355209751203No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC227000.15483166183346334No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA164350.11209948732303833No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT148230.10110439309944613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC285400.042.97240445
CGTATGC286550.042.7829344
CTCGTAT289000.042.17111642
TCGTATG291350.041.9313643
GCACACG312000.041.2147611
ACGTCTG312950.041.1778915
TCTCGTA299650.040.6800841
GTCACAC301200.040.58582329
CACACGT317300.040.5192412
CACGTCT319050.040.50952514
ACACGTC317550.040.5024813
CAGTCAC302850.040.46156727
AGCACAC317800.040.42496510
CGTCTGA320200.040.25959416
CCAGTCA304850.040.1813526
AGTCACA305000.039.88863428
CTGAACT323050.039.79025719
TCCAGTC322300.039.44948625
GAGCACA326000.039.4413079
AATCTCG307800.039.29580739